Vitamin D3

2,000 IU

UK adults are systematically deficient. D3 (cholecalciferol) raises 25-OH-D levels 3× more effectively than D2. Required for calcium absorption, immune modulation, and muscle function. SACN recommends 400 IU as the reference nutrient intake; 2,000 IU is a meaningful therapeutic dose.

Category Micronutrients
Dose 2,000 IU
Form Cholecalciferol - lanolin-derived
Evidence Level Strong - extensive human data, SACN reviewed

Mechanism

Vitamin D3 (cholecalciferol) is the form synthesised in skin under UVB radiation. It requires hepatic hydroxylation to 25-OH-D3, then renal hydroxylation to 1,25-dihydroxyvitamin D3 (calcitriol) - the active hormone that binds vitamin D receptors (VDR) in virtually every tissue. VDR-mediated signalling regulates calcium and phosphate homeostasis, immune function, muscle protein synthesis, and gene expression across hundreds of genes.

UK Public Health England data shows that ~40% of adults have 25-OH-D levels below 25 nmol/L in winter and spring. SACN (2016) set the RNI at 400 IU/day to prevent deficiency - but this is a threshold dose, not an optimal dose. Studies targeting 25-OH-D levels above 75 nmol/L - associated with optimal immune and musculoskeletal function - require 1,500-2,000 IU/day supplementation in most UK adults.

Key Benefits

  • Required for calcium and phosphate absorption
  • VDR-mediated immune modulation across virtually every tissue
  • Supports muscle protein synthesis and function
  • ~40% of UK adults deficient in winter - supplementation essential
  • 2,000 IU matches the dose used in published clinical research

The Research

Peer-reviewed human trials supporting this ingredient at this dose.

Holick MF. (2007). N Engl J Med. 357(3):266-81.Vitamin D deficiency.PubMed →
Tripkovic L, et al. (2012). Am J Clin Nutr. 95(6):1357-64.Comparison of vitamin D2 and vitamin D3 supplementation in raising serum 25-hydroxyvitamin D status: a systematic review and meta-analysis.PubMed →
Pludowski P, et al. (2013). Autoimmun Rev. 12(10):976-89.Vitamin D effects on musculoskeletal health, immunity, autoimmunity, cardiovascular disease. A review.PubMed →
Pilz S, et al. (2011). Horm Metab Res. 43(3):223-5.Effect of vitamin D supplementation on testosterone levels in men.PubMed →
Martineau AR, et al. (2017). BMJ. 356:i6583.Vitamin D supplementation to prevent acute respiratory tract infections: systematic review and meta-analysis of IPD.PubMed →
In the formula

Vitamin D3

2,000 IU

Cholecalciferol - lanolin-derived

When to take it

Take with food containing fat - D3 is fat-soluble. Morning is preferred by some to support circadian rhythm. 2,000 IU is within the upper safe limit (4,000 IU per SACN 2016).