Why 100mcg of Biotin?

How Biotin works

Biotin is covalently attached to all four human biotin-dependent carboxylase enzymes: pyruvate carboxylase (gluconeogenesis), acetyl-CoA carboxylase (fatty acid synthesis), propionyl-CoA carboxylase (odd-chain fatty acid catabolism), and 3-methylcrotonyl-CoA carboxylase (leucine catabolism). These enzymes collectively regulate glucose production, fatty acid metabolism, and amino acid breakdown.

Beyond metabolism, biotin also functions in epigenetic regulation - it is covalently attached to histones by biotinidase and holocarboxylase synthetase, affecting chromatin structure and gene expression. The UK NRV of 0.5mcg is a minimum deficiency threshold; the 50mcg dose in this formula targets the tissue saturation levels studied in hair and nail keratin synthesis trials.

Key benefits

• Obligate covalent cofactor for all four carboxylase enzymes
• Required for de novo fatty acid synthesis
• Supports gluconeogenesis (glucose production)
• Histone biotinylation - epigenetic gene regulation
• 50mcg = 100× UK NRV

The researchGB-NHC

Peer-reviewed human trials supporting this ingredient at this dose.

1. Zempleni J, et al. (2009). Biofactors. 35(1):36-46.Biotin.PubMed →
2. Patel DP, et al. (2017). Skin Appendage Disord. 3(3):166-9.A review of the use of biotin for hair loss.PubMed →
3. Jungert A, et al. (2022). Eur J Nutr. 61(4):1779-87.Revised D-A-CH reference values for the intake of biotin.PubMed →

Works well with